TCB Publications - Abstract

U. Mirsaidov, J. Comer, V. Dimitrov, A. Aksimentiev, and G. Timp. Slowing the translocation of double-stranded DNA using a nanopore smaller than the double helix. Nanotechnology, 21:395501-10, 2010.

MIRS2010-AA It is now possible to slow and trap a single molecule of double-stranded DNA (dsDNA), by stretching it using a nanopore, smaller in diameter than the double helix, in a solid-state membrane. By applying an electric force larger than the threshold for stretching, dsDNA can be impelled through the pore. Once a current blockade associated with a translocating molecule is detected, the electric field in the pore is switched in an interval less than the translocation time to a value below the threshold for stretching. According to molecular dynamics (MD) simulations, this leaves the dsDNA stretched in the pore constriction with the base-pairs tilted, while the B-form canonical structure is preserved outside the pore. In this configuration, the translocation velocity is substantially reduced from 1 bp/10 ns to $\sim$1 bp/2 ms in the extreme, potentially facilitating high fidelity reads for sequencing, precise sorting, and high resolution (force) spectroscopy.

Request Full Text

Request Paper

Full Name
Email Address
Type the number eight in the box