Jiankuai Diao and Emad Tajkhorshid.
Indirect role of Ca2+ in the assembly of extracelluar matrix
proteins.
Biophysical Journal, 95:120-127, 2008.
(PMC: 2426659)
DIAO2008-ET
We have investigated through molecular dynamics the binding properties at the interface between the C-type lectin subdomain (CLD) of aggrecan and the fibronectin type III domains (FnIII) of tenasin, in
particular the mechanistically unknown, but essential role of Ca ions in the binding. The binding between the CLD and FnIII is critical in cross-linking aggrecan, hyaluronan and tenascin to form extended protein networks found in tissues such as cartilage. None of the structurally resolved ions in the complex of the CLD and FnIII is directly bridging the two proteins. However, one of the Ca ions (Ca2) is found to play the role of maintaining the structure of the L4 loop at the CLD binding surface, thus facilitating a high affinity binding between the CLD and FnIII. Removal of this Ca ion causes a drastic structural change in the L4 loop, which presumably hinders the binding of the CLD to the FnIII. The other bound Ca ions (Ca1 and Ca3) have no significant effect on the structure of the CLD
binding surface, and thus are not expected to affect the binding. Our results might also suggest that the role of Ca2 in maintaining the structure of the L4 loop is most important during the binding process.
Once the complex is formed, the dependence of the complex on the structuring role of Ca2 is reduced. In response to tensile force, CLD and FnIII separate by breaking first the electrostatic interactions at the interface, followed by the hydrophobic ones. The sequence of the unbinding events and the maximum force required to
separate the two proteins are independent of the presence of the ions, underlying the indirect role of Ca in the binding.
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