Yi Chen, Eduardo R. Cruz-Chu, Jaie Woodard, Manas Ranjan Gartia, Klaus
Schulten, and Logan Liu.
Electrically induced conformational change of peptides on metallic
nano-surfaces.
ACS Nano, 6:8847-8856, 2012.
(PMC: 3482133)
CHEN2012
Surface immobilized biomolecular probes are used in many areas of biomedical
research, such as genomics, proteomics, immunology, and pathology. Although the
structural conformations of small DNA and peptide molecules in free solution is well
studied both theoretically and experimentally, the conformation of small biomolecules
bound on surfaces, especially under the influence of external electric fields, is poorly
understood. Using a combination of molecular dynamics simulation and surface enhanced
Raman spectroscopy, we study the external electric field-induced conformational
change of dodekapeptide probes tethered to a nanostructured metallic surface. Surface-tethered peptides with and without phosphorylated tyrosine residues are compared
to show that peptide conformational change under electric field is sensitive to
biochemical modification. Our study proposes a highly sensitive in vitro nanoscale
electro-optical detection and manipulation method for biomolecule conformation and
charge at bio-nano interfaces.
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